Composite

Part:BBa_K2442406

Designed by: Ruiyang He, James Provan   Group: iGEM17_Glasgow   (2017-09-07)

[split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator

This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-GFP AND-gate


This is a composite of the GFP C-term split subunit (GFP2) K2442400, the well characterised double-terminator construct B0015, and the well characterised lacI regulated promoter R0011


Usage and Biology

this composite was used for assembly of larger composite split-GFP AND-gate constructs.

  • K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 264


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Parameters
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